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Detection of Milk Pasteurisation Using ALP Testing KIT

Milk is an essential source of nutrients, vitamins, carbohydrates, minerals and fats. Pasteurization of milk is one important aspect to maintain its quality over a period of time as it kills the bacteria present in it.

At times the commercial milk packets are not pasteurized. So to test whether the milk is pasteurized or not?. Scientists at IIT Guwahati have developed an alkaline phosphatase or ALP milk testing kit using a simple filter paper.

Detection of Milk Pasteurisation Using ALP Testing KIT

Milk is an important source of nutrients and vitamins required for the development of the human body. It provides several benefits from early lactation by providing immunological support to old age acts as a source of calcium.

Being an animal product, The shelf life of milk is very less. As a lot of microorganisms can grow fast & spoil its quality and nutritive value. To make milk microorganism free and maintain its nutritive value a technique called pasteurization is used.

Detection of Milk Pasteurisation

Pasteurization is a process in which milk is heated to a particular temperature to kill harmful bacteria followed by rapid cooling. It is always advisable to consume pasteurized milk for Better Health. Alkaline phosphatase or ALP is an enzyme which is naturally presented milk, but it is destroyed on the temperature just near to the pasteurization temperature.

So the presence of alkaline phosphatase in milk can be a good indicator for the quality of pasteurization. Several methods are time-consuming and require sophisticated instrumentation are used for this purpose, which also restricts on-site testing the quality of milk.

Using ALP Testing KIT

To test the quality of pasteurization and milk, scientists at the Indian Institute of Technology Guwahati have developed a low cost and environment-friendly & on-site detection kit based on biosensor technology. This kit has shown its usefulness in detecting ALP level in commercial as well as raw milk samples.

The researchers at IIT Guwahati under the supervision of Dr Pranjal Chandra (Assistant Professor) developed a biosensor detector using normal filter paper discs, soaked with some bacterial chemical to react it with ALP. Upon adding the milk sample, The paper disc changes its colour from white to Blue. If the milk is not pasteurized the amount of alkaline phosphatase present in the milk sample can be Quantified.

Key Points

  • Pasteurisation is used to make milk free from the microorganism.
  • Alkaline phosphatase is an enzyme which is naturally present in the milk.
To maintain the quality of milk pasteurization process, paper-based miniaturized Immuno sensor developed by  Dr Pranjal Chandra and his research team is a great work and is the development is going to be beneficial for the commercial industry as well as for on-site detection of possible contamination in processed and raw milk.